primary antibodies against mitf  (Santa Cruz Biotechnology)

Journal: Cell Death & Disease

Article Title: Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma

doi: 10.1038/s41419-024-06733-3

Figure Lengend Snippet: A Representative Western Blot images depicting murine MITF and BRN2 protein levels in primary AP2ε -/- /Tg(GRM1) and Tg(GRM1) cells (left panels) and human MITF and BRN2 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX (right panels). β-Actin served as loading control. B Immunohistochemical staining of MITF and BRN2 protein expression in Mel Im melanoma cells bioprinted in CIB (Magnification: 20x). Quantification of percentage of cells with “no”, “weak”, or “strong” MITF and BRN2 expression. Data are represented as mean ± SEM; * p < 0.05; ns : not significant (Two-way-ANOVA with Fisher’s LSD test). C mRNA- and protein expression analysis for Snail1 in AP2ε -/- /Tg(GRM1) and Tg(GRM1) cells ( n = 3). D mRNA- and protein expression analysis for Snail1 in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX ( n = 3). E mRNA- and protein expression analysis for E-cadherin in AP2ε -/- /Tg(GRM1) and Tg(GRM1) cells ( n = 3). F mRNA- and protein expression analysis for E-cadherin in Mel Juso cells transfected with the AP2ε-overexpression (hAP2ε) plasmid and control vector pCMX ( n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05 (Two-tailed Student’s t-test; ns : not significant).

Article Snippet: Primary antibodies against MITF (1:500 in 5% BSA, Santa Cruz, sc-515925, RRID:AB_2828036), BRN2 (1:500 in 5% BSA/1x TBS-T, Santa Cruz, sc-393324, RRID:AB_2737347), Snail1 (1:1,000 in 5% BSA/1x TBS-T, Cell Signaling Technology Cat# 3879, RRID:AB_2255011) and E-cadherin (1:1,000 in 5% BSA/1x TBS-T, Cell Signaling Technology Cat# 3195, RRID:AB_2291471) were incubated overnight at 4 °C.

Techniques: Western Blot, Transfection, Over Expression, Plasmid Preparation, Control, Immunohistochemical staining, Staining, Expressing, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: EGCG, GCG, TFDG, or TSA Inhibiting Melanin Synthesis by Downregulating MC1R Expression

doi: 10.3390/ijms241311017

Figure Lengend Snippet: Effect of EGCG, GCG, TFDG, or TSA on expression of TYR, tyrosinase-related protein 1 (TRP1), and tyrosinase-related protein 2 (TRP2) mRNA and protein. B16F10 cells were pretreated with EGCG, GCG, TFDG, or TSA for 2 h and stimulated with α-MSH for 24 h in the presence of EGCG, GCG, TFDG, or TSA. The treatment group without α-MSH and a sample was referred to as CK1, and the treatment group with α-MSH but without a sample was referred to as CK2. ( a ) Cell lysates were prepared with an EASY spin Plus cell RNA rapid extraction kit. Total RNAs were subjected to qPCR analysis of TYR , TRP1, or TRP2 with β-actin as an internal control. Data are mean ± SD. ( b ) Protein extracts were prepared by lysing cells with radioimmunoprecipitation assay (RIPA) lysate containing protease inhibitors and phosphatase inhibitors. Total proteins were subjected to Western blot analysis of TYR, TRP1, or TRP2 with GAPDH as an internal control. Data are mean ± SEM; a,b,c,d,e,f,g different letters above the column indicate significant differences ( p < 0.05).

Article Snippet: Primary antibodies against TYR (TU343218), TRP1 (TD13325), TRP2 (TA5303), MITF (TU326886), p-MITF (Ser180, TA3027), and MC1R (TD4992) were purchased from Abmart (Shanghai, China).

Techniques: Expressing, Radio Immunoprecipitation, Western Blot